Sample Prep

Sample Prep

DNA Extraction

DNA Extraction

Our DNA Extrac­tion ser­vice pre­pares high qual­ity DNA sam­ples from blood (up to 10 ml) only. The DNA samples will be returned to you for storage, processing or shipment to collaborators.

Price:  See Pricing page

CONTACT

IHGSeqCore@ucsf.edu

Exome Capture

Exome Capture

To capture the coding regions of the genome

We can build a customized project for you based on our consultant rate and pass-through supplies. Please contact us at the email below.

Details: Libraries are pooled into a capture reaction that contains biotinylated DNA oligonucleotides (called ‘baits’), from Nimblegen, (SeqCap EZ Human Exome Library v3.0; Roche Nimblegen) for 72 hours. The DNA bait-DNA hybrids are then pulled out of the complex mixture by incubation with streptavidin-labeled magnetic beads and captured onto a strong magnet. After washing, the targeted DNA of interest is eluted and subjected to 18 cycles of DNA amplification.
Vendor Website: Nimblegen
Price: See Pricing page

CONTACT

IHGSeqCore@ucsf.edu

Library Prep

Library Prep

Construction of libraries for Illumina sequencing

We can build a customized project for you based on our consultant rate and pass-through supplies. Please contact us at the email below.

Details: Genomic DNA sheared by Covaris S2 to a target size of 200-300bp and assembled into a library with TruSeq adapters containing indexes that differentiates different libraries in a capture reaction as well as a sequencing run.
Vendor Website: Kapa Biosystems
Price: See Pricing page

CONTACT

IHGSeqCore@ucsf.edu

Consultation

Genomics Consultation

Please call us to discuss any projects that require custom design and specialized services

 

Price:  See Pricing page

 

CONTACT

IHGSeqCore@ucsf.edu