Whole Exome Sequencing
LIBRARY PREPARATION: Construction of libraries for Next-Generation Sequencing
We can build a customized project for you based on our consultant rate and pass-through supplies. Please contact us at the email below.
Details
The genomic DNA is sheared by the Covaris LE220 to a target insert size of ~350bp and assembled into a library constructed with unique dual indexes which are compatible with the NovaSeq.
Vendor: Roche
EXOME CAPTURE
To capture the protein coding region of the human genome
Details
Libraries are pooled into a capture reaction that contains biotinylated oligonucleotide probes to target specific regions of interest. The biotinylated probe/target hybrids are pulled down by streptavidin-coated magnetic beads to obtain libraries highly enriched for the target regions.
Vendor: Roche
DATA PROCESSING & ANALYSIS
Complimentary sequencing data will be provided in the fastq format. Under certain circumstances, sequence alignment to a reference genome and variant calling can be provided for an additional charge. Consultation for the clinical genomic variant is also available for an additional charge. All additional services are subjected to availability. Please contact us for further information.